Stem cell frequency in nanofat adipose tissue and its association with clinical variables
Keywords:
Adipose tissue; Mesenchymal stem cells; Adipose tissue-derived stem cells; Stromal vascular fraction; Flow cytometry.Abstract
Introduction: Autologous adipose tissue processed with nanofat is a biomaterial widely used in regenerative therapy, primarily due to its stem cell content. It is necessary to investigate whether certain clinical characteristics of the patient are associated with the frequency of these cell populations.
Objective: To expose the frequency of stem cell populations in adipose tissue processed with nanofat, as well as their association with clinical variables.
Methods: A cross-sectional descriptive study was conducted on 111 patients (114 samples) treated with nanofat lipotransfer at the Plastic Surgery and Burn Treatment Service of the “Hermanos Ameijeiras” Clinical Surgical Hospital. The patients were characterized, and the cell populations in the adipose tissue were identified. The Mann-Whitney U and Kruskal-Wallis tests were applied to evaluate associations (α = 0.05).
Results: The median expression rates were: CD34 2.94%, ASCs 8.56%, MSCs 1.54 %, and VSELs 0.68 %. Patients with acute burns showed the highest frequencies of CD34 (5.59 %; p = 0.015) and ASCs (18.97 %; p = 0.036). The abdominal region as a donor site was associated with higher CD34 expression (3.22 % vs. 1.60 %; p = 0.049).
Conclusions: The expression frequency of surface markers in mesenchymal stem cell populations, adipose-derived stem cells, and very small embryonic stem cells from nanofat processed adipose tissue varies according to the patient's clinical characteristics, allowing for the optimization of patient selection for regenerative therapies.
Downloads
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2026 Alicia María Tamayo Carbón, Arturo Chang Monteagudo, Elizabeth Hernández Ramos; Susel Quesada Peña; Diana Katherine Cuastumal Figueroa
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Aquellos autores/as que tengan publicaciones con esta revista, aceptan los términos siguientes:
- Los autores/as conservarán sus derechos de autor y garantizarán a la revista el derecho de primera publicación de su obra, el cuál estará simultáneamente sujeto a la Licencia de reconocimiento de Creative Commons que permite a terceros compartir la obra siempre que se indique su autor y su primera publicación esta revista.
- Los autores/as podrán adoptar otros acuerdos de licencia no exclusiva de distribución de la versión de la obra publicada (p. ej.: depositarla en un archivo telemático institucional o publicarla en un volumen monográfico) siempre que se indique la publicación inicial en esta revista.
- Se permite y recomienda a los autores/as difundir su obra a través de Internet (p. ej.: en archivos telemáticos institucionales o en su página web) antes y durante el proceso de envío, lo cual puede producir intercambios interesantes y aumentar las citas de la obra publicada. (Véase El efecto del acceso abierto).
